Fig. 5

The impact of histone demethylase inhibition on the migration, invasive potential, and epithelial-mesenchymal transition of cervical cancer cells was studied. A The results of the wound healing assay, which measures cell migration. The scale bar is 50 μm, and B the outcomes of the Transwell chamber experiments, which assess cell invasion. The scale bar is 12.5 μm. C The results of Western blot analysis, evaluating the effects of histone demethylase inhibition on cell migration, invasion, and the expression of EMT-related proteins. D The impact of histone demethylase inhibition on global H3K27me3 and H3K27me1 in HeLa and SiHa cells, as determined by Western blot. E The changes in the enrichment of H3K27me3 binding to the promoter regions of target genes were determined using ChIP-qPCR (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, compared to DMSO Ctrl