Fig. 6

ACTA2-AS1 increased KLF9 expression and inhibited the invasive ability of PTC cells in a miR-4428-dependent manner. A qRT-PCR was employed to determine the KLF9 mRNA level in TPC1 cells overexpressing ACTA2-AS1 and miR-4428. B, C, D, E Western blot and quantitative analysis, respectively, of the KLF9 protein level in TPC1 cells overexpressing ACTA2-AS1 and miR-4428. F, G Transwell invasion assay and quantitative analysis, respectively, for the invasive ability of TPC1 cells overexpressing ACTA2-AS1 and miR-4428. Scale bar, 50 µm and magnification, 200× . **p < 0.01