Fig. 5

In vivo and in vitro ARTs have distinct effects on genome-wide DNA methylation in cord blood. A Total number of hypomethylated and hypermethylated DMCs between in vivo/in vitro and control groups. B Methylation differences at DMCs between in vivo/in vitro and control groups, with pie charts summarizing the proportion of DMCs demonstrating less than 10%, between 10 and 20% and greater than 20% methylation differences. C Distribution of DMCs within genomic regions (left), repetitive elements (middle) and CpG-rich regions (right) in comparison to all captured sites genome-wide. D Pie charts demonstrating the categorical distribution of all significant biological processes enriched within genic DMCs for the in vivo/in vitro versus control analysis. Selected significant biological processes from Gene Ontology analysis are shown for the in vitro versus control analysis. The number of observed genes associated with each term is indicated within bars. Enrichment is defined as the proportion of observed genes compared to the number annotated within the whole dataset and is indicated by bar colour. The dotted line represents the significance threshold: weighted Fisher’s p value < 0.01