Fig. 3From: Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2AZM attenuates overexpression of P65, EZH2 and H3K27me3 and cytokines and alleviates wheezing time. A–C The expression levels of P65, EZH2 and H3K27me3 were examined by Western blotting (n = 4). D–G Effects of AZM on IL-2, IL-6, IL-10 and INF-γ in the BALF (n = 3). H Three shEZH2 intervention sites were used to optimize the transfection efficiency, and that of the lentiviral vector was analyzed by immunofluorescence containing EZH2 shRNA. Scale bars indicate 100 μm. I Lentiviral transfection efficiency of shEZH2 was analyzed by Western blotting. #P < 0.05 versus the shCtrl group (n = 3). J, K The expression of EZH2 and H3K27me3 after shEZH2 intervention was detected by protein immunoblotting. &P < 0.05 versus the Wheeze + shCtrl group (n = 3). L Effects of AZM on wheezing time in patients. M Effects of mycoplasma infection on AZM and wheezing time. *P < 0.05 versus the Wheeze groupBack to article page