Fig. 2

Methylation of LINC00473 in colorectal cancer tissues. Methylation analysis of LINC00473 promoter in tissues from primary CRC and matched normal colorectal mucosa (controls) of two independent cohorts analyzed by 450K array (Cohort 1) and pyrosequencing (Cohort 2). A, B Methylation status of LINC00473 in (A) all CRC patients (N = 293) and (B) in those CRC patients (N = 283) with available clinical information on their TNM tumor stage (Cohort 1). C ROC curve analysis evaluating the methylation of LINC00473 for the detection of CRC in tissue samples in Cohort 1. D, E Validation of the methylation status of LINC00473 in (A) all CRC patients (N = 180) and (B) in those CRC patients (N = 178) with available clinical information on their TNM tumor stage in Cohort 2. F ROC curve analysis to validate the methylation of LINC00473 for the detection of colorectal tumors in Cohort 2. Horizontal lines represent mean methylation levels of LINC00473. P, p value analyzed by Mann–Whitney U test or ROC curve; AUC, area under the ROC curve; Ctrl, controls; CRC, colorectal cancer