Methylation status of nc886 epiallele reflects periconceptional conditions and is associated with glucose metabolism through nc886 RNAs

Table 1 KEGG and BIOCARTA pathways associated with nc886-3p and -5p expression

Gene set name	# Genes in gene set (K)	# Genes in Overlap (k)	k/K	p value	FDR q value
nc886-3p
KEGG_ENDOCYTOSIS	181	8	0.044	4.37 × 10^–6	0.002
KEGG_FC_GAMMA_R_MEDIATED_PHAGOCYTOSIS	96	6	0.063	9.99 × 10^–6	0.002
KEGG_CHRONIC_MYELOID_LEUKEMIA	73	5	0.069	3.62 × 10^–5	0.004
KEGG_INSULIN_SIGNALING_PATHWAY	137	6	0.044	7.46 × 10^–5	0.005
KEGG_FOCAL_ADHESION	199	7	0.035	7.51 × 10^–5	0.005
nc886-5p
KEGG_INOSITOL_PHOSPHATE_METABOLISM	54	6	0.111	3.53*10^–7	1.68 × 10^–4
KEGG_PHOSPHATIDYLINOSITOL_SIGNALING_SYSTEM	76	6	0.079	2.72 × 10^–6	0.001
KEGG_ACUTE_MYELOID_LEUKEMIA	57	5	0.088	1.13 × 10^–5	0.002
Both nc886-3p and nc886-5p
KEGG_ACUTE_MYELOID_LEUKEMIA	57	7	0.123	1.23 × 10^–6	0.001
BIOCARTA_ERK_PATHWAY	27	5	0.185	5.36 × 10^–6	0.001
KEGG_INOSITOL_PHOSPHATE_METABOLISM	54	6	0.111	1.31 × 10^–5	0.002
KEGG_FC_GAMMA_R_MEDIATED_PHAGOCYTOSIS	96	7	0.073	4.04 × 10^–5	0.004
KEGG_INSULIN_SIGNALING_PATHWAY	137	8	0.058	5.66 × 10^–5	0.004
KEGG_PATHWAYS_IN_CANCER	325	12	0.037	8.20 × 10^–5	0.004
KEGG_PHOSPHATIDYLINOSITOL_SIGNALING_SYSTEM	76	6	0.079	9.28 × 10^–5	0.004
KEGG_FOCAL_ADHESION	199	9	0.045	1.42 × 10^–4	0.006
KEGG_HEMATOPOIETIC_CELL_LINEAGE	87	6	0.069	1.96 × 10^–4	0.008
KEGG_ENDOCYTOSIS	181	8	0.044	3.85 × 10^–4	0.012
KEGG_CHRONIC_MYELOID_LEUKEMIA	73	5	0.069	0.001	0.021
BIOCARTA_MAPK_PATHWAY	81	5	0.062	0.001	0.028
KEGG_REGULATION_OF_ACTIN_CYTOSKELETON	213	8	0.038	0.001	0.028
KEGG_CHEMOKINE_SIGNALING_PATHWAY	189	7	0.037	0.002	0.044

Gene set enrichment was analyzed for genes that were predicted targets of nc886-3p/nc886-5p according to mircoRNA.org and whose RNA levels correlated (spearman rank order correlation) with the targeting ncRNA at the level of p < 0.05. Pathways with FDR < 0.05 and more than five genes overlapping are presented in the table

ISSN: 1868-7083