Fig. 6
From: Diuron exposure and Akt overexpression promote glioma formation through DNA hypomethylation
APOBEC3γ promotes the PD-L1 and Bcl-w demethylation, while the DNMT1/PCNA/UHRF1 disruption promotes the LLT1 demethylation. qMSRE (as previously described) compare the relative methylation level of Bcl-w, LLT1, and PD-L1 genes in cells transiently transfected with the pcDNA.3.1 (Ctrl), pcDNA.3.1-UP plasmid, and with pcDNA.3.1-APOBEC3γ plasmids. pcDNA.3.1-UP is a plasmid encoding for a chimeric protein having the ability to selectively disrupt the DNMT1/UHRF1 interaction [3, 4]