Fig. 3From: Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genesBisulfite pyrosequencing of candidate gene GTF2H4 (cg22508626) validates direction of methylation change identified in 450k array. a–c Univariate analysis of GTF2H4 cg22508626 in Illumina 450k BeadChip array. a Methylation β values for AMD (n = 25) compared to normal RPE donor cells (n = 19) after normalization, in addition to analysis of sex-stratified results for cg22508626 (GTF2H4). Significantly increased methylation levels are observed in AMD compared to normal (p = 0.0003), in addition to both b AMD male (n = 15) and c AMD female (n = 10) compared to normal male (n = 12) (p = 0.0074) and normal female (n = 7) (p = 0.0136) human donor samples respectively. d–f Bisulfite pyrosequencing of candidate gene GTF2H4 (cg22508626) in combined technical and independent sample replications. d Mean methylation difference observed in cg22508626 (GTF2H4) in AMD (n = 29) compared to normal donor samples (n = 24) does not reach statistical significance (p = 0.1263). Sex-stratified analysis reveals no significant methylation differences in e AMD male (n = 18) versus normal male (n = 18) donor samples. Significantly increased methylation is observed in f AMD female (n = 11) versus normal male (n = 6) (p = 0.0292). (*p ≤ 0.05)(**p ≤ 0.01)(***p ≤ 0.001)(****p ≤ 0.0001). All statistical analysis was performed using the Mann-Whitney U testBack to article page