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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Retinoic acid-induced 2 (RAI2) is a novel tumor suppressor, and promoter region methylation of RAI2 is a poor prognostic marker in colorectal cancer

Fig. 4

The effect of RAI2 on colorectal cancer migration and invasion. a Migration assay results showing the number of migrated cells before and after the restoration of RAI2 expression in RKO and LOVO cells as well as in the control group (shNC), shRAI2 knockdown group, and shRAI2 knockdown plus MK2206 treated group (*P < 0.05, **P < 0.01). b Invasion assay results showing the number of invasive cells before and after re-expression of RAI2 in RKO and LOVO cells as well as in the control group (shNC), shRAI2 knockdown group, and shRAI2 knockdown plus MK2206 treated group (***P < 0.001). c The expression levels of E-cadherin, vimentin, pan-AKT, p-AKTser473, MMP-2, MMP-7, MMP-9, and actin (control) were detected by Western blot in RAI2 un-expressed and re-expressed RKO and LOVO cells as well as in the control group, shRAI2 knockdown group, and shRAI2 knockdown plus MK2206 treated group (ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001).

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Clinical Epigenetics

ISSN: 1868-7083

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