Fig. 2

DNA methylation changes in human hematopoietic cells upon stable engraftment into mice. a Scatterplot of DNAm levels in humanized mice (HuMice) versus CD34⁺ cord blood (CB) samples. Significant hyper- and hypomethylation is highlighted in red and blue, respectively (delta of mean β values > 0.2 or < − 0.2; adjusted limma P value < 0.05). CpG sites that are associated with promoter regions (located in the 5′ untranslated region (5′ UTR), 200 bp (TSS200), and 1500 bp (TSS1500) upstream of transcription start site) [24] are more likely to be reflected in differential gene expression and were therefore highlighted in bold (2425 CpGs and 169 CpGs, respectively). b Gene ontology (GO) analysis of genes associated with differentially methylated CpG sites in promoter regions (one-sided Fisher’s exact P value). The most significant categories are exemplarily depicted (categories comprising more than 1000 genes were not considered and similar categories are only listed once)