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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis

Fig. 1

Confirmation of the primers designed for calculating integration in the Caski cell line. PCR products were electrophoresed on two 1.5% agarose gels that were run at the same time. The primer used is indicated above the band. DL 5000 was used as the reference marker (Takara, Japan), with bands from the top to the bottom representing 5000, 3000, 2000, 1000, 750, 500, 250, and 100 bp, respectively. DNA bands that were not the expected size are shown in a small box, and these fragments were sent for direct sequencing. There were some recurrent interruptions, and the different band sizes occurred in the same lane

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