Fig. 8
From: Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure
Effects of PBN on fractional synthesis of histones in frontal cortex of PF and AF rat pups. We measured the incorporation of 2H in the canonical histone peptides H3 (kQLATkAAR), H4 (gkGGkGLGkGGAkR) and histone variant peptides H3.3 (kSAPSTGGVkkPHR), H2Az (aTIAGGGVIPHIHkSLIGkkGQQkTV), and H2Ax (kGHYAER) in frontal cortex of 6-day-old rat pups following a 5-day alcohol feeding and administration of 2H2O and 100 mg/Kg of the free radical spin trap PBN (α-phenyl-N-tert-butyl nitrone). Histone Fractional Synthesis Rate (FSR) was calculated as described in the “Methods” section, and data are shown as mean ± SE, n = 5–8 per group, *p < 0.05, **p < 0.01, AF vs PF, #p < 0.05, ##p < 0.01, AF-PBN vs PF, AF and PF-PBN