Fig. 1

mPCR uses HpaII restriction digestion, two-color PCR and CE for determination of size and methylation. a Schematic diagram of restriction sites in the 5’ UTR of the FMR1 gene. The HpaII sites flank the CGG repeat region. Arrows indicate location of the forward (mPCR Fwd) and reverse (mPCR Rev) PCR primers. b mPCR assay workflow. Percent methylation can be calculated from the normalized ratio of peak heights in the HEX and FAM channels