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Fig. 2 | Clinical Epigenetics

Fig. 2

From: MSP-HTPrimer: a high-throughput primer design tool to improve assay design for DNA methylation analysis in epigenetics

Fig. 2

The MSP-HTPrimer sequence: the principle of in silico deamination workflow. The MSP-HTPrimer pipeline takes into account that deamination of double-stranded native DNA (left) results in four different template sequences (right) for primer design. Thus, from the plus and minus strand, different methylation-dependent primer pairs can be designed (M - methylated; U - unmethylated; Cs in blue - not in a CpG context are usually unmethylated)

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