Molecular Pap smear: HPV genotype and DNA methylation of ADCY8, CDH8, and ZNF582 as an integrated biomarker for high-grade cervical cytology

Table 1 Clinical and cytological characteristics of the study population

Characteristics	Number	Percentage^g
Clinical
Age^a
Median (IQR)	28	(24–35)
Range (year)	19–67
Race/ethnicity^a
Asian [NILM, LSIL, HSIL]	9 [5, 3, 1]	(3)
Black [NILM, LSIL, HSIL]	40 [14, 15, 11]	(14)
White [NILM, LSIL, HSIL]	124 [48, 40, 36]	(45)
Other [NILM, LSIL, HSIL]	65 [21, 28, 16]	(24)
Unknown [NILM, LSIL, HSIL]	39 [12, 14, 13]	(14)
Cytological^b
Total LBC samples collected	400	(100)
LBC samples excluded^c	123	(31)
NILM	33
LSIL	85
HSIL	5
LBC samples included	277	(69)
NILM	100
LSIL	100
HSIL	77
Source^d
Cervical	276	(99.6)
Vaginal	1	(0.4)
Diagnostic category^d
Normal	100	(36)
Abnormal	177	(64)
Cellular DNA concentration^d,e,f
NILM	100
Median (ng/μL) (IQR)	48.1	(37.1–74.3)
Range (ng/μL)	20.8–181.5
LSIL	100
Median (ng/μL) (IQR)	46.3	(35.6–63.1)
Range (ng/μL)	20.5–182.8
HSIL	77
Median (ng/μL) (IQR)	51.8	(34.9–71.3)
Range (ng/μL)	11.5–154.0

IQR interquartile range, LBC liquid-based cytology, LSIL low-grade squamous intraepithelial lesion, HSIL high-grade squamous intraepithelial lesion, NILM negative for intraepithelial lesion and malignancy
^aAge and race/ethnicity of subjects (N = 277) are based on the demographic data of included samples. The number of samples by race/ethnicity and cytological diagnosis are placed in square brackets. The distribution of the five categories of race/ethnicity (inclusive of Other and Unknown) among the three cytological grades were not significantly different (χ ², p = 0.777)
^bCytopathology results are ascribed to the specimens collected on the day of the study enrollment
^cExclusion criteria included: low cell pellet volume (<200 μL); low cellular DNA concentration (<20 ng/μL); low nucleic acid purity (spectrophotometry absorbance ratio 260/230 nm <0.7); excess samples (>100) (see text for details)
^dData based on included samples (N = 277)
^eConcentration of total cellular DNA per sample after manual or semi-automated DNA extraction
^fComparison of DNA concentrations between NILM, LSIL, and HSIL samples were not significantly different (p = 0.519) by Kruskal-Wallis test
^gValues are N (%) unless otherwise denoted

ISSN: 1868-7083