Table 1 Demographic and pathological features of the study cases
Training set | DNA validation set (MSP and pyroseq) | RNA validation set (qPCR) | Protein validation set (IHC) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
SAC | CC | SAC | CC | SAC | CC | SAC | CC | |||||
n = 39 (%) | n = 34 (%) | p value | n = 59 (%) | n = 44 (%) | p value | n = 18 (%) | n = 25 (%) | p value | n = 42 (%) | n = 49 (%) | p value | |
Gender | ||||||||||||
Female | 20 (51.3) | 16 (47.1) | 29 (49.2) | 21 (47.7) | 8 (44.4) | 9 (36.0) | 30 (71.4) | 28 (57.1) | ||||
Male | 19 (48.7) | 18 (52.9) | 0.719 | 30 (50.8) | 23 (52.3) | 0.846 | 10 (55.6) | 16 (64.0) | 0.5763 | 12 (28.6) | 21 (42.9) | 0.157 |
Age [SD] | 71.6 [10.0] | 72.3 [7.6] | 72.0 [9.5] | 70.3 [8.4] | 0.348 | 69.4 [9.8] | 72.6 [11.0] | 0.331 | 69.9 [6.8] | 70 [10.7] | 0.959 | |
Location | ||||||||||||
Proximal | 26 (66.7) | 21 (61.8) | 34 (57.6) | 26 (59.1) | 10 (55.6) | 14 (56.0) | 23 (54.8) | 26 (53.1) | ||||
Distal /rectum | 13 (33.3) | 13 (44.1) | 0.554 | 25 (42.3) | 18 (40.1) | 0.882 | 8 (44.4) | 11 (44.0) | 0.977 | 19 (45.2) | 23 (46.9) | 0.871 |
Dukes’ stage | ||||||||||||
A | 4 (10.3) | 4 (11.8) | 6 (10.2) | 4 (9.1) | 2 (11.1) | 3 (12.0) | 7 (16.7) | 8 (16.3) | ||||
B | 13 (33.3) | 15 (44.1) | 24 (40.7) | 18 (40.9) | 4 (22.2) | 13 (52.0) | 15 (35.7) | 17 (34.7) | ||||
C | 17 (43.6) | 15 (44.1) | 0.875 | 29 (49.2) | 22 (50.0) | 0.983 | 12 (66.7) | 9 (36.0) | 0.094 | 20 (47.6) | 24 (49.0) | 0.992 |
WHO grade | ||||||||||||
High | 5 (12.8) | 2 (5.9) | 3 (5.1) | 1 (2.3) | 0 | 0 | 0 | 2 (4.1) | ||||
Low | 34 (87.2) | 32 (94.1) | 0.315 | 56 (94.9) | 43 (97.3) | 0.465 | 18 (100) | 25 (100) | NA | 42 (100) | 47 (95.9) | 0.186 |
Type | ||||||||||||
Non-mucinous | 34 (87.1) | 30 (88.2) | 51 (84.4) | 39 (88.6) | 16 (88.9) | 22 (88.0) | 36 (58.7) | 41 (83.7) | ||||
Mucinous | 5 (12.8) | 4 (11.8) | 0.891 | 8 (13.6) | 5 (11.4) | 0.740 | 2 (11.1) | 3 (12.0) | 0,929 | 6 (14.3) | 8 (16.3) | 0.788 |