Fig. 4

Functional analysis reveals links between DMRs and aging, neurodevelopment, and calcium transport. a Bar diagram shows differential methylation between Hi and Lo in the 100 strongest peaks previously reported as either hyper- or hypomethylated in aged fibroblast cells [34]. b Shows the normalized distribution of disease classes associated with the genes located closest to the p < 0.0001 DMRs (n = 407). Normalization was done to control for publication bias, by dividing the number of DMR genes associated with a disease class with the total number of genes present within that same class. c Heatmap showing the same subset of the DMRs as in b. Columns represent normalized counts of each child and are ordered by hair cortisol rank. Rows represents median centered Z-scores of individual DMRs, where the row order has been determined by hierarchical cluster analysis (euclidean distance; also presented as a dendogram to the right). d Cluster analysis of DMRs associated with genes involved in axon guidance (GO:0007411). Red indicates a significantly robust cluster determined by bootstrap resampling. Hi high hair cortisol, Lo low hair cortisol, PSY psychology, NEU neurological, CHE chemical dependence, VIS vision, AGE aging, CAD cardiovascular, HEM hematological, REN renal, MET metabolic, IMM immune, INF infection, CAN cancer, DEV development, REP reproduction, PHA pharmacogenomic, OTH other