Figure 6From: Coordinated epigenetic remodelling of transcriptional networks occurs during early breast carcinogenesisThe methylation profile of vHMEC hypermethylated loci can identify tumour from normal in the TCGA breast cancer cohort. (A) HM450 probes overlapping with vHMEC DMR chr19-37205 are hypermethylated in tumours in the TCGA-BRCA cohort. (B) DMR chr19-37205 overlaps the COX7A1 promoter associated CpG island. Methylation at the locus was able to separate tumour from normal tissue in the TCGA-BRCA cohort (AUC > 0.94). (C) vHMEC DMR chr5-12238 overlapped two HM450 probes; however, in the TCGA-BRCA cohort, several adjacent probes also exhibited increased methylation. (D) Hypermethylation of DMR chr5-12238 overlapping and adjacent probes (covering the LCT4S promoter) was specific to tumours in the TCGA-BRCA cohort (ROC analysis AUC > 0.97). Additionally, a small independent cohort of HM27 profiled samples also exhibited tumour-specific methylation at this locus (AUC = 0.94).Back to article page