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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2

Fig. 5

AZM and SN50 inhibit NF-κB P65 nuclear translocation induced by LPS in rat alveolar macrophages. A AZM and SN50 suppressed LPS-induced NF-κB P65 nuclear translocation in rat alveolar macrophages (NR8383). Immunofluorescence staining showed NF-κB P65 localization in NR8383 cells. The scale bars represent 50 μm. B Interaction of H3K27me3, EZH2 and P65 was detected by Co-IP in NR8383. Co-IP of cell lysates of NR8383 treated as indicated with anti-H3K27me3-protein G magnetic beads and immunoblot (IB) with an anti-EZH2 and anti-NF-κB P65 antibody for evaluation of protein expression. IgG: negative control. C Co-IP quantification analysis. #P < 0.05 versus the Control group, *P < 0.05 versus the LPS group (n = 3)

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Clinical Epigenetics

ISSN: 1868-7083

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