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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Epigenetic dysregulation-mediated COL12A1 upregulation predicts worse outcome in intrahepatic cholangiocarcinoma patients

Fig. 6

COL12A1 promotes iCCA cell growth. A CRISPR/Cas9-mediated COL12A1 knockout efficiency in HuCCT1 or RBE cells was evaluated by immunoblotting and RT-PCR, respectively. B The impact of COL12A1 knockout on HuCCT1 or RBE cells proliferation was evaluated by CCK8 assay. C Representative images of colony-forming assays and the colony numbers in HuCCT1 or RBE cells with or without COL12A1 knockout. D Representative gross images of subcutaneous xenograft tumor with or without COL12A1 knockout. Xenograft tumor volumes and weights were analyzed between the two groups (n = 10 mice). E Representative images of H&E and Ki67 staining of subcutaneous xenograft tumor. Original magnification: 10/20X. Bar plot to show Ki67 positive cells from 5 different fields in xenograft tumor slides of the two groups. Experiments were in triplicates. *p < 0.05, **p < 0.01, ****p < 0.0001, using two-tailed unpaired Student's t test. iCCA intrahepatic cholangiocarcinoma

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