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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Epigenetic dysregulation-mediated COL12A1 upregulation predicts worse outcome in intrahepatic cholangiocarcinoma patients

Fig. 2

COL12A1 is upregulated in clinical iCCA tissues and human iCCA cells. A Box plots showing COL12A1 mRNA levels in iCCA and adjacent nontumor liver tissues from TCGA, GEO or ArrayExpress datasets, respectively. **p < 0.01; ****p < 0.0001, using two-tailed unpaired Student's t test. B COL12A1 expression levels in iCCA, HCC and the paired nontumor liver tissues were determined by immunoblotting and RT-PCR, respectively. ns, p ≥ 0.05. *p < 0.05, using two-tailed paired Student's t test except for the comparison of the relative COL12A1 protein level between iCCA and HCC tissues using Wilcoxon test. C COL12A1 expression pattern in human iCCA and normal human intrahepatic biliary cell (HIBEpiC) lines were determined by immunoblotting and RT-PCR, respectively. ****p < 0.0001, using one-way analysis of variance (ANOVA). D Representative images of H&E and COL12A1 staining in 60 pairs of iCCA samples. Boxplot showing COL12A1 protein levels in iCCA and the paired nontumor liver tissues. ****p < 0.0001, using two-tailed paired Student's t test. E Representative images of immunofluorescence staining for COL12A1 and DAPI in the indicated human iCCA cells. Original magnification: 100X; scale bar = 20 μm. Experiments were triplicated. iCCA, intrahepatic cholangiocarcinoma; HCC, hepatocellular carcinoma; GEO, Gene Expression Omnibus; TCGA, The Cancer Genome Atlas; RT-PCR, real-time PCR; T, tumor tissue; N, paired nontumor liver tissue, m: protein maker (ladder)

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