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Fig. 6 | Clinical Epigenetics

Fig. 6

From: HDAC2 enhances esophageal squamous cell carcinoma development through down-regulating microRNA-503-5p and promoting CXCL10

Fig. 6

CXCL10 is positively connected with HDAC2 and negatively connected with miR-503-5p in ESCC. a TargetScan software to analyze the binding site of miR-503-5p and CXCL10; b dual-luciferase reporter gene assay to analyze the binding relation between miR-503-5p and CXCL10; c RT-qPCR to detect CXCL10 mRNA expression in ESCC cells after miR-503-5p expression regulation; d Western blot assay to detect CXCL10 protein expression in ESCC cells after miR-503-5p expression regulation; e RT-qPCR to detect CXCL10 mRNA expression in ESCC cells after HDAC2 expression regulation; f Western blot assay to detect CXCL10 expression in ESCC cells after HDAC2 expression regulation; g RT-qPCR to detect CXCL10 protein expression in ESCC cancer and normal esophageal epithelial tissues; h Pearson analysis to assess the correlation between miR-503-5p and CXCL10 in ESCC cancer tissues; i Pearson analysis to assess the correlation between CXCL10 and HDAC2 in ESCC cancer tissues; for all quantitative results, the data were expressed as mean ± standard deviation of three independent experiments. Discrepancies between two groups were assessed by Student’s t test while those among multiple groups by one-way ANOVA; in figure bd, * P < 0.05 compared with the miR-503-5p-NC group. In figure e/f, * P < 0.05 compared with the sh-NC group

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