Fig. 4

HDAC2 is bound to miR-503-5p promoter and inhibits miR-503-5p expression. a. ChIP assay to detect HDAC2 enrichment on the miR-503-5p promoter in ESCC cells; b. RT-qPCR to detect miR-503-5p expression in ESCC cells after HDAC2 knockdown; c. RT-qPCR to detect miR-503-5p mRNA expression in ESCC cell lines and normal epithelial cells; d. RT-qPCR to detect miR-503-5p expression in ESCC cancer and normal esophageal epithelial tissues; e. Pearson analysis to assess the correlation between HDAC2 and miR-503-5p in ESCC cancer tissues; for all quantitative results, the data were expressed as mean ± standard deviation of three independent experiments. Discrepancies between two groups were assessed by Student’s t test while those among multiple groups by one-way ANOVA; * P < 0.05 compared with HEEC cells