Clinical Epigenetics

Table 1 iPLEX MassArray confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples

From: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

Gene	450K array data				iPLEX MassArray data
	Allergy	Control	Delta beta	p _adj ^a	Allergy	Control	Delta beta	p ^b	p ^c
	Beta ± SD	Beta ± SD	Delta beta	p _adj ^a	Beta ± SD	Beta ± SD	Delta beta	p ^b	p ^c
ALOX12	0.35 ± 0.10	0.39 ± 0.07	− 0.044	0.0001	0.24 ± 0.08	0.26 ± 0.05	− 0.021	0.109	0.102
APOBEC1	0.84 ± 0.09	0.86 ± 0.07	− 0.022	0.0042	0.76 ± 0.23	0.83 ± 0.20	− 0.068	0.109	0.102
*GLI2*	0.74 ± 0.10	0.73 ± 0.07	0.009	< 0.0001	0.63 ± 0.12	0.61 ± 0.09	0.020	0.034*	0.035*
*GRAMD1B*	0.16 ± 0.04	0.15 ± 0.03	0.010	0.0023	0.10 ± 0.03	0.08 ± 0.03	0.016	0.002*	0.002*
*HTRA3*	0.66 ± 0.07	0.65 ± 0.05	0.013	0.0091	0.56 ± 0.07	0.53 ± 0.06	0.030	0.036*	0.011*
*MED24*	0.53 ± 0.02	0.54 ± 0.02	− 0.011	0.0055	0.40 ± 0.04	0.40 ± 0.03	− 0.001	0.328	0.036*
OPCML	0.66 ± 0.07	0.65 ± 0.09	0.003	0.0006	0.50 ± 0.08	0.48 ± 0.12	0.021	0.125	0.140

^aCorrected for estimated cell counts (according to Houseman et al.), batch, and gender and adjusted for multiple testing
^bCorrected for batch and gender
^cCorrected for estimated buccal and granulocyte cell fractions, batch, and gender
Genes given in bold are significantly differentially methylated (*p <0.05)

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