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Fig. 2 | Clinical Epigenetics

Fig. 2

From: KAT3B-p300 and H3AcK18/H3AcK14 levels are prognostic markers for kidney ccRCC tumor aggressiveness and target of KAT inhibitor CPTH2

Fig. 2

CPTH2 modifies stress fiber organization and cell morphology of ccRCC 786-O cell line. a Cytoskeleton organization stained with phalloidine showed a drastic reorganization of stress fibers, adhesion placks, and cell morphology after 48 h of CPTH2 (100 μM) treatment with respect to untreated and DMSO samples. b Percentage of stress fibers/cell after 18 h of CPTH2 treatment compared to DMSO sample. c Percentage of 786-O cell adhesion (%) after 12 and 24 h CPTH2 treatment with respect to DMSO tested on different substrates, BSA, fibronectin (FN), matrigel (Matr.), and collagen (Coll). d mRNA expression of modulators of adhesion and migration CD44, and integrins ITGb3, ITGb1, ITGa5, and ITGa6 analyzed by qRT-PCR, shows remarkable similarity with an increase after 24/48 h of CPTH2 treatment. e Scratch test confirmed a strong inhibition of cell migration and invasiveness after 48 h of CPTH2 treatment compared to DMSO control. f Percentage of migrating (cells/mm2) and invading cells (cells/mm2) after 24 and 48 h of CPTH2 (black) treatment with respect to DMSO (light gray)

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