Fig. 6From: Pharmacological inhibition of EZH2 disrupts the female germline epigenomeTazemetostat depleted H3K27me3 in primary oocytes. Confocal images of H3K27me3 (red) and DAPI (blue) immunofluorescence in sections of E18.5 mouse ovaries cultured for 7 days with either DMSO (top panel) or 5 μM tazemetostat (bottom panel; 10 μm scale bars). Graphical representation of mean H3K27me3 staining intensity in DMSO treatment in green and tazemetostat treatment in blue, analysed by ROI manager, ImageJ. P < 0.005, Student’s t test; DMSO n = 4 and tazemetostat n = 3 biological replicates, three primary oocytes analysed per replicate. Error bars ± SEMBack to article page