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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Pharmacological inhibition of EZH2 disrupts the female germline epigenome

Fig. 4

EZH2 inhibition potently reduced H3K27me3 levels in fetal oocytes undergoing epigenetic reprogramming. a. Flow scatter plots showing H3K27me3 mean intensity of germ cells measured by flow cytometry of XX E12.5 gonads cultured for 48 h with DMSO, 100 nM, 300 nM or 1 μM tazemetostat. Examples of flow cytometric gating are shown in Additional file 1: Figure S1. b Average H3K27me3 staining intensity in germ cells from XX female E12.5 gonads cultured for 48 h with DMSO, 100 nM, 300 nM, 1 μM tazemetostat. ****P < 0.0001, one-way ANOVA plus post hoc Tukey’s multiple comparisons test; n = 3–5 biological replicates. Error bars ± SEM. c Cell cycle state in germ cells from XX female E12.5 gonads cultured for 72 h with DMSO, 1 μM and 5 μM tazemetostat. No significant differences between DMSO control and treatments. One-way ANOVA plus post hoc Tukey’s multiple comparisons test; n = 3–5 biological replicates. Error bars ± SEM

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