Fig. 3

H3K27me3 was not recovered in oocytes of adult mice after tazemetostat treatment. Confocal images of immunofluorescence in ovary sections from adult mice treated. a Treatment for 10 days with vehicle control (sham; 0.5% NaCMC, 0.1% Tween 80). b Treatment for 10 days with 220 mg/kg tazemetostat injected daily. c. Treatment for 10 days with vehicle control (sham; 0.5% NaCMC, 0.1% Tween 80) followed by a 10-day no treatment recovery period. d. Treatment for 10 days with 220 mg/kg tazemetostat followed by a 10-day no treatment recovery period. Each panel shows merged colour images with H3K27me3 shown in red and DAPI (DNA) in blue. Oocyte nuclei are shown within the white dashed line. Images are representative of three biological replicates. 10 μm scale bars. e. Mean fluorescence intensity for H3K27me3 for each treatment, analysed by ROI manager, ImageJ. ****P < 0.0001 one-way ANOVA plus post hoc Tukey’s multiple comparisons test; n = 4 biological replicates, 7–12 oocytes measured per replicate. Error bars ± SEM