Fig. 2
From: Pharmacological inhibition of EZH2 disrupts the female germline epigenome
Tazemetostat treatment of adult female mice substantially reduced H3K27me3 in growing oocytes. Confocal images of immunofluorescence in ovary sections from adult mice treated for 10 days with vehicle control (sham; 0.5% NaCMC, 0.1% Tween 80) or 220 mg/kg tazemetostat injected daily. Each panel shows merged colour images with H3K27me3 shown in red and DAPI (DNA) in blue. Oocyte nuclei are shown within the white dashed line. Images are representative of three biological replicates. 10 μm scale bars. Mean fluorescence intensity for H3K27me3 is shown in the graph to the right analysed by ROI manager, ImageJ. ****P < 0.0001 one-way ANOVA plus post hoc Tukey’s multiple comparisons test; n = 4 biological replicates per treatment, 5–7 oocytes measured per replicate. Error bars ± SEM