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Table 5 The fluorescence peak height of a dilution of methylated template in the background of unmethylated template and their correlation coefficiency of different gene standards for methylation analysis of HRM-PCR

From: Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis

Genes Standards
Fluorescence peak height Correlation coefficient
0% 1% 25% 50% 75% 100%
ESBSs 69.20 51.00 42.50 33.60 21.90 5.80 0.963
L1-1 76.10 42.20 35.70 24.50 14.80 5.10 0.971
L1-2 66.20 46.70 38.20 24.00 12.30 5.80 0.983
L1-3 56.30 48.80 40.20 28.50 12.30 3.60 0.976
L1-4 77.80 47.60 32.80 25.40 47.20 7.20 0.919
L1-5 89.40 70.90 55.00 39.00 15.00 6.90 0.953
L1-6 66.90 49.30 32.10 25.00 12.30 2.50 0.989
L1-7 60.40 55.00 41.60 18.00 12.00 2.70 0.963
L2-1 70.30 51.90 45.00 32.50 19.30 6.10 0.967
L2-2 69.80 44.80 32.10 22.30 10.50 1.60 0.985
L2-3 65.00 35.40 23.00 21.10 13.20 2.10 0.984
L2-4 67.50 47.70 39.00 25.90 12.30 2.10 0.959
L2-5 70.30 41.50 30.00 21.30 15.00 2.70 0.978
E2 66.00 55.90 42.10 23.80 12.20 2.50 0.950
E6 68.90 47.20 35.70 30.10 24.50 7.90 0.987
E7 66.70 55.90 48.10 26.70 25.20 5.80 0.958