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Fig. 1 | Clinical Epigenetics

Fig. 1

From: DNA methylation as a predictor of fetal alcohol spectrum disorder

Fig. 1

Visualization and verification of the differentially methylated probes. a Heatmap of the 161 validated probes validated in the KBHN cohort at an FDR < 0.05 (79 hypermethylated in FASD; 82 hypomethylated in FASD). The percent methylation values (ranging from 0 to 100) were centered, scaled, and trimmed, resulting in a standardized DNA methylation level ranging from − 2 to + 2 (blue-red scale). b Scatter plot of the differences in percent methylation between FASD and controls for the 648 differentially probes identified in the NDN cohort. The mean differences between groups were highly correlated between both the NDN and KBHN cohorts (r = 0.638, p < 2.2e-16). The red points show the probes that were statistically significant (FDR < 0.05) and showed the same direction of change across both studies c Verification by bisulfite pyrosequencing in FASD (blue) and control (gray) samples. The left panel shows the DNA methylation levels from the pyrosequencing assay, while the right panel shows the results from the 450K array. The CpG assayed was located in the CACNA1A gene body (cg24800175) and showed statistically significant differences between groups (p = 0.04)

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