Fig. 4
Changes in expression of apoptotic regulators and cellular signaling after combined treatment with Romidepsin and JQ1. Relative mRNA expression levels of antiapoptotic BCL-XL (a) and BCL-2 (b) and proapoptotic BCL-XS (c). mRNA levels were measured by qRT-PCR and normalized to the expression of TBP. Fold change compared to DMSO control 48 h after treatment is displayed on the ordinate. Differences between combination treatment samples and untreated controls were analyzed using Student’s t test (**p ≤ 0.01, *p ≤ 0.05). d Protein expression of antiapoptotic BCL-XL and Survivin after combination treatment for 48 h assessed by Western blot. e Protein expression of c-MYC, EZH2, p57KIP2, and phospho-p57KIP2 (Thr310) assessed by Western blot analysis. f Phosphorylated AKT (S473) and expression of AKT and SKP2 was detected by Western blot analysis