Fig. 4

KIBRA expression is activated by demethylation using 5-azacytidine. a DNA demethylation by 5-azacytidine (Aza) and inhibition of histone deacetylases by trichostatin A (TSA) resulted in ~3-fold increase in KIBRA mRNA levels in neuronal SH-SY5Y cells. Cells were treated with 5 μM Aza for 3 days and 250 nM TSA for the last 24 h. KIBRA expression levels were analyzed by PCR and subsequent densitometry compared to hRP27 control. b Effect of DNA demethylation by Aza and inhibition of histone deacetylases by TSA on KIBRA protein expression detected by western blot analysis. KIBRA protein levels increased significantly after treatment with Aza and Aza/TSA. Figures are representative for experiments (n ≥ 3); western blot densitometry is based on three individual experiments. **p < 0.01, ***p < 0.001, ns not significant