Fig. 4
From: Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers
Measurement of 5hmC using commercial ELISA kits. a Quest 5-hmC™ DNA ELISA Kit. Bar graphs representing 5hmC levels in colon and peripheral blood DNA samples measured at two average fragment lengths. DNA fragments >30 kbp (green bars) and DNA fragments 500 bp in length (yellow bars). Different fragment lengths for the same DNA sample produced significantly different results. b, c MethylFlash™ Hydroxymethylated DNA Quantification Kit. b Bar graph comparison of 5hmC level as calculated by the kit for healthy blood (n = 1) and CLL (n = 1). c Bar graph comparison of 5hmC level as calculated by the kit for healthy colon (n = 2), adjacent tissue (n = 2), and CRC (n = 2). Both absolute and relative 5hmC levels are inconsistent with published data and the measurements presented in this study