Fig. 2
From: Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers
Illustration of sample imaging and analysis process. a Two-colored DNA molecules are stretched on glass slides. b Multiple fluorescence microscope images are obtained per sample, showing both YOYO-1 labelling of the entire molecule (red) and the Cy5 labelling of 5hmC-modified nucleobases (green/yellow dots). c User interface of the image processing software, where the length of the DNA molecules is measured (green) and collocalized 5hmC (red dots) is detected and subsequently quantified relative to DNA length. d Output of the statistics tool, showing the total length of DNA (in pixels, X axis) and the percentage of 5hmC relative to DNA (%5hmC, Y axis). e Comparison of %5hmC of the total sample data with two randomly divided subsets of this data which contain similar amount of DNA