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Table 2 Comparison of mRNA levels of selected genes (median ∆Cq and median fold change) as assessed by real time RQ-PCR in CLL (n = 93; unmutated = 39; mutated = 54) and CD19+ sorted B-cells from healthy individuals (n = 10)

From: Genome-wide DNA methylation profiling integrated with gene expression profiling identifies PAX9 as a novel prognostic marker in chronic lymphocytic leukemia

Comparison of levels of mRNA expression of selected genes in CLL
S.No. Gene CLL Vs. normal   Mutated CLL Vs. unmutated CLL
   Group Median ∆Cq Median fold change p value IGHV Mutation status Median ∆Cq Median fold change p value
1. CRY1 CLL 8.61 0.75 0.91 Mutated 9.8 0.31 <0.001
19+ Normal 8.29 Unmutated 7.46 1.73
2. MEIS1 CLL 13.03 0.07 0.01 Mutated 12.85 0.05 0.85
19+ Normal 8.87 Unmutated 12.63 0.06
3. ID4 CLL 13.75 4.37 0.03 Mutated 13.51 3.89 0.45
19+ Normal 15.7 Unmutated 13.71 3.2
4. TNRC18 CLL 13.21 0.64 0.43 Mutated 12.93 0.62 0.6
19+ Normal 12.53 Unmutated 13.34 0.59
5. NFATC1 CLL 8.43 0.5 0.47 Mutated 8.86 0.39 0.37
19+ Normal 7.54 Unmutated 8.37 0.57
6. CDK6 CLL 12.65 0.2 0.02 Mutated 12.65 0.25 0.7
19+ Normal 10.48 Unmutated 12.65 0.26
7. VIPR1 CLL 6.62 7.9 0.04 Mutated 6.5 6.96 0.32
19+ Normal 9.34 Unmutated 5.98 11.4
8. SPRY1 CLL 12.75 0.04 <0.001 Mutated 12.18 0.05 0.28
19+ Normal 8.49 Unmutated 12.7 0.03
9. PAX9 CLL 12.29 0.81 0.66 Mutated 12.8 0.37 <0.001
19+ Normal 11.61 Unmutated 9.28 4.61
10. PMEPA CLL 12.33 0.01 <0.001 Mutated 12.32 0.01 0.47
19+ Normal 5.52 Unmutated 11.72 0.01
11. TBX2 CLL 16.92 0.1 0.004 Mutated 16.42 0.08 0.67
19+ Normal 13.45 Unmutated 16.91 0.08
12. TSHZ3 CLL 10.9 1.19 0.81 Mutated 10.57 1.15 0.56
19+ Normal 10.97 Unmutated 11.18 0.82
13. BIK CLL 8.27 0.4 0.5 Mutated 8.4 0.44 0.54
19+ Normal 7.26 Unmutated 8.08 0.53
14. SPRY2 CLL 10.2 6.2 0.02 Mutated 9.78 6.59 0.42
19+ Normal 12.4 Unmutated 10.26 4.6
15. AXIN2 CLL 9.8 6.3 0.23 Mutated 9.2 4.79 0.56
19+ Normal 11.77 Unmutated 8.91 6.66
16. SOX7 CLL 11.93 0.18 0.02 Mutated 11.65 0.18 0.99
19+ Normal 9.23 Unmutated 11.92 0.16
17. RIC8B CLL 8.77 1.85 0.38 Mutated 8.59 1.85 0.78
19+ Normal 9.61 Unmutated 8.72 1.75
  1. The statistically significant p values are shown in italics