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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Methylation of DIRAS1 promotes colorectal cancer progression and may serve as a marker for poor prognosis

Fig. 4

The function of DIRAS1 in DIRAS1 highly expressed HCT116 cells and in vivo study of DIRAS1 in colorectal cancer cell xenograft mice. a Growth curves represent cell viability analyzed and by the MTT assay in DIRAS1 highly expressed HCT116 cells before and after knockdown of DIRAS1. The experiments were performed in triplicate. (***P < 0.001). b Colony formation assays show colony numbers in DIRAS1 highly expressed HCT116 cells before and after knockdown of DIRAS1. Each experiment was repeated three times, c the average number of tumor clones is represented by bar diagram. (**P < 0.01). d Cell migration and invasion results in DIRAS1 highly expressed HCT116 cells before and after knockdown of DIRAS1, e ratios are presented by bar diagram. Each experiment was repeated three times. (**P < 0.01, ***P < 0.001). f The expression levels of DIRAS1, MMP2, and MMP9 were detected by Western blot in DIRAS1 highly expressed HCT116 cells before and after knockdown of DIRAS1. Knockdown of DIRAS1 by siRNA was performed to validate the results in DIRAS1 highly expressing HCT116 cells. siNC DIRAS1 highly expressed HCT116 cells, siDIRAS1 knockdown of DIRAS1 in HCT116 cells. g Volumes of xenograft tumors in DIRAS1 re-expressed and unexpressed in DLD1 cells after 2 and 3 weeks, respectively (*P < 0.05, **P < 0.01). h Weights of xenograft tumors in DIRAS1 re-expressed and unexpressed DLD1 and RKO cells after incubation for 3 weeks (**P < 0.01). i IHC shows the expression of DIRAS1 in DIRAS1 re-expressed and unexpressed DLD1 cell xenografts (×400)

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