Table 4 Primer and probe sequences of the quantitative methylation (QM) real-time PITX3 PCR
Primer/probe name | Primer/probe sequence |
|---|---|
PITX3-F | 5′-CTCTCACAACACAACTCCTATTC-3′ |
PITX3-R | 5′-TTTAGGTTTAGATTTTTGGGGTT-3′ |
PITX3-P-M | 5′-VIC-CGACCAAACGCACCCCG-BHQ-2-3′ |
PITX3-P-U | 5′-FAM-ATACAACCAAACACACCCCAACTCC-BHQ-1-3′ |