Skip to main content

Table 1 Tobacco smoking-associated methylation change at single CpG in different leukocyte populations of the blood

From: Tobacco smoking differently influences cell types of the innate and adaptive immune system—indications from CpG site methylation

Gene

AHRR

CPOX

F2RL3

GFI1

GPR15

PAICS

PAK2

CpG

cg05575921

cg02657160

cg03636183

cg09935388

cg19859270

cg13086586

cg02319016

Leukocyte population

 Whole blood (n = 10, each group)

  NS (%)a

89.8 ± 5.9

85.5 ± 2.7

77.1 ± 8.6

66.6 ± 4.9

93.9 ± 1.3

12.7 ± 1.6

91.3 ± 3.7

S (%)a

68.2 ± 8.3

83.4 ± 2.0

71.8 ± 6.9

62.2 ± 3.7

92.9 ± 1.0

13.9 ± 1.9

90.0 ± 2.2

  ∆meth (%)d

−21.6 (−14.8; −28.4)

−2.1 (0.1; −4.3)

−5.3 (0.4; −10.9)

4.4 (−0.3; −8.5)

1.0 (0.1; −2.1)

1.2 (2.8; −0.4)

−1.3 (1.6; −4.2)

p valueb

2.8E−04

6.0E−02

6.6E−02

3.6E−02

4.7E−02c

1.4E−01

3.5E−01

 Granulocyte (n = 10, each group)

  NS (%)a

85.2 ± 7.3

85.3 ± 1.4

75.4 ± 5.5

52.9 ± 5.8

96.0 ± 0.5

12.4 ± 1.3

93.0 ± 1.5

S (%)a

30.0 ± 7.0

84.6 ± 1.7

66.7 ± 4.2

41.7 ± 7.3

95.8 ± 0.6

11.8 ± 2.8

92.3 ± 2.5

  ∆meth (%)d

55.2 (−48.5; −61.9)

−0.7 (0.8; −2.2)

8.7 (−3.9; −13.5)

11.2 (−5.0; −17.4)

−0.2 (0.3; −0.7)

−0.6 (1.5; −2.7)

−0.7 (1.2; −2.6)

p valueb

1.2E−12

3.3E−01

1.3E−03

1.3E−03

4.3E−01c

1.0E+00c

4.5E−01

 PBMC (n = 6, each group)

  NS (%)a

92.2 ± 3.3

84.3 ± 1.6

82.0 ± 3.2

86.2 ± 2.9

93.0 ± 1.1

11.3 ± 3.1

87.2 ± 3.2

S (%)a

76.5 ± 5.2

80.0 ± 2.2

84.5 ± 8.1

83.8 ± 3.2

88.8 ± 3.1

12.5 ± 3.0

86.7 ± 0.5

  ∆meth (%)d

15.7 (−10.0; −21.3)

4.3 (−1.8; −6.8)

2.5 (10.4; −5.4)

−2.3 (1.6; −6.2)

4.2 (−1.2; −7.1)

1.2 (5.1; −2.8)

−0.5 (2.4; −3.4)

p valueb

5.0E−03c

3.0E−03

5.0E−01

2.1E−01

9.4E−03c

4.6E−01c

3.7E−01c

 CD3+GPR15− T cell (n = 6, each group)

  NS (%)a

93.7 ± 2.1

84.5 ± 1.4

85.5 ± 6.6

87.7 ± 3.7

95.2 ± 1.5

11.7 ± 3.3

83.0 ± 3.5

S (%)a

90.0 ± 2.2

81.3 ± 2.7

86.0 ± 6.8

88.5 ± 2.1

92.7 ± 2.0

13.2 ± 5.2

80.7 ± 4.2

  ∆meth (%)d

3.7 (−0.9; −6.4)

3.2 (−0.4; −5.9)

0.5 (9.1; −8.1)

0.8 (4.7; −3.0)

2.5 (−0.3; −4.7)

1.5 (7.1; −4.1)

−2.3 (2.7; −7.3)

p valueb

1.4E−02

2.7E−02

9.0E−01

6.4E−01

3.2E−02

5.6E−01

3.2E−01

 CD3+GPR15+ T cell (n = 6, each group)

  NS (%)a

91.3 ± 1.9

74.8 ± 3.8

83.7 ± 6.6

87.5 ± 1.4

55.2 ± 9.4

12.8 ± 3.4

78.5 ± 4.8

S (%)a

78.7 ± 5.0

59.0 ± 1.3

86.2 ± 6.8

88.7 ± 2.5

41.3 ± 7.4

17.2 ± 8.6

68.5 ± 6.4

  ∆meth (%)d

12.7 (−7.8;−17.6)

15.8 (−12.2;−19.4)

2.5 (11.1;−6.1)

1.2 (3.8;−1.4)

13.8 (−3.0;−24.7)

4.3 (12.7;−4.0)

10.0 (−2.7;−17.3)

p valueb

1.8E−04

2.0E−06

5.1E−01c

3.4E−01

1.8E−02

2.2E−01c

1.2E−02

  1. Significant differences between smokers (S) and non-smokers (NS) are in italics (p < 0.05)
  2. aMean
  3. bStudent’s t test
  4. cMann-Whitney U test
  5. dLimit of 95 % confidence interval in parentheses