Insulin-like growth factor axis in pregnancies affected by fetal growth disorders

Clinical Epigenetics

Table 2 Correlation between DNA methylation and mRNA expression

Gene	CpG number	CpG location	Rs	p value
IGF1	1	Chr12: −102874567	−0.6275	0.007*
IGF1	2	Chr12: −102874689	−0.7377	0.0007*
IGF1	3	Chr12: −102874755	−0.4044	0.10
IGFBP1	11	Chr7: 45927583	−0.4728	0.01*
IGFBP1	12	Chr7: 45927579	−0.6349	0.0005*
IGFBP1	13	Chr7: 45927575	−0.7005	<0.0001*
IGFBP2	9	Chr2: 217497928	−0.7785	<0.0001*
IGFBP2	10	Chr2: 217497846	−0.5856	0.001*
IGFBP2	11	Chr2: 217497740	−0.6205	0.0007*
IGFBP3	46	Chr7: −45960433	−0.8086	<0.0001*
IGFBP3	47	Chr7: −45960418	−0.6627	0.0002*
IGFBP3	48	Chr7: −45960415	−0.5104	0.007*
IGFBP3	49	Chr7: −45960400	−0.4161	0.04*
IGFBP3	50	Chr7: −45960394	−0.6306	0.001*
IGFBP4	23	Chr17: 38599317	−0.5971	0.002*
IGFBP4	24	Chr17: 38599314	−0.3923	0.05
IGFBP4	25	Chr17: 38599311	0.04471	0.83
IGFBP4	26	Chr17: 38599308	−0.6726	0.0003*
IGFBP4	27	Chr17: 38599305	0.626	0.001*
IGFBP4	28	Chr17: 38599302	−0.5158	0.009*
IGFBP4	29	Chr17: 38599299	−0.1613	0.45*
IGFBP7	8	Chr4: −57976141	−0.6501	0.0006*
IGFBP7	9	Chr4: −57976145	−0.6882	0.0002*
IGFBP7	10	Chr4: −57976152	0.19	0.37
IGFBP7	11	Chr4: −57976157	−0.2764	0.19
IGFBP7	12	Chr4: −57976172	−0.05846	0.78

Spearman’s correlation between mRNA expression and DNA methylation showed significant inverse correlation at all CpGs tested except in two locations (24 and 25) at IGFBP4 promoter region and at three locations (10, 11 and 12) at IGFBP7 promoter region

ISSN: 1868-7083