Maternal B vitamins: effects on offspring weight and DNA methylation at genomically imprinted domains

Clinical Epigenetics

Table 4 Adjusted regression coefficients for maternal B vitamins and infant differentially methylated regions: Newborn Epigenetic STudy (N = 429)

	H19 DMR	MEG3 DMR	SGCE/PEG10 DMR	PLAGL1 DMR
	mean methylation % (standard deviation)
	47.93 (3.82)	72.64 (5.55)	45.98 (5.17)	57.79 (6.33)
Maternal B vitamins	β coefficient, Standard Error, p value
B₁₂
≤322.47 ng/L	Reference	Reference	Reference	Reference
322.48–446.04 ng/L	0.53, 0.57, 0.35	0.53, 0.85, 0.53	0.01, 0.66, 0.98	0.39, 0.95, 0.68
446.05–575.51 ng/L	0.68, 0.56, 0.22	0.01, 0.82, 0.99	0.26, 0.66, 0.70	0.58, 0.94, 0.54
>575.51 ng/L	−0.41, 0.57, 0.48	−0.93, 0.85, 0.27	0.47, 0.67, 0.48	1.79, 0.96, 0.06
PLP
≤3.76 nM/L	Reference	Reference	Reference	Reference
3.77–7.47 nM/L	−0.15, 0.60, 0.80	0.23, 0.83, 0.79	0.99, 0.75, 0.19	−0.02, 0.98, 0.99
7.48–12.05 nM/L	−0.68, 0.62, 0.28	2.01, 0.89, 0.03	−0.33, 0.79, 0.68	−0.26, 1.02, 0.80
>12.05 nM/L	−0.07, 0.63, 0.91	3.24, 0.89, <0.01	−0.30, 0.81,0.71	−0.11, 1.04, 0.91
PA
≤2.06 nM/L	Reference	Reference	Reference	Reference
2.07–3.21 nM/L	−0.45, 0.58, 0.44	−0.18,0.84, 0.83	1.46, 0.74, 0.05	1.27, 0.95,0.18
3.22–5.93 nM/L	−0.09, 0.60, 0.88	−0.24, 0.87, 0.79	0.79, 0.75, 0.29	1.54, 0.98, 0.12
>5.93 nM/L	−0.57, 0.61,0.35	1.62, 0.87, 0.06	0.08, 0.78, 0.92	−0.15, 0.99, 0.88
Hcy
≤4.40 umol/L	Reference	Reference	Reference	Reference
4.41–5.10 umol/L	1.01, 0.59, 0.09	1.49, 0.87, 0.09	1.43, 0.77, 0.06	1.46, 0.98, 0.14
5.11–6.00 umol/L	−0.97, 0.58, 0.10	1.07, 0.86, 0.21	1.19, 0.76, 0.12	1.77, 0.97, 0.07
>6.00 umol/L	0.19, 0.60, 0.75	1.60, 0.87, 0.07	1.36, 0.79, 0.09	1.10, 0.98, 0.27

Adjusted for gestational age at delivery, gestational age at blood draw, maternal race/ethnicity, maternal smoking and pre-pregnancy body mass index
DMR differentially methylated region, B ₁₂ vitamin B₁₂, PLP pyridoxal phosphate, PA 4-pyridoxic acid, Hcy homocysteine

ISSN: 1868-7083