Fig. 4

SFN decreased HDAC3 protein expression in colon tumors of Nrf2^+/+ but not Nrf2^−/+ mice. a HDAC expression was immunoblotted in tumor (T) and adjacent normal-looking colon (N) of WT mice, with densitometry data normalized to β-actin. b The corresponding data for Nrf2^−/+ mice. Data are given as mean ± SD (n = 3); *P < 0.05, **P < 0.01 for control diet vs. SFN treatment. In addition to global reductions in HDAC3, marked loss of HDAC3 on gene targets was examined (see Fig. 6)