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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Nucleated red blood cells impact DNA methylation and expression analyses of cord blood hematopoietic cells

Fig. 6

Erythroid-WBC interactions during FACS affect DNAm based on the proportion of nRBCs. As illustrated, only a fraction of erythroid cells are nucleated; therefore, contaminating nRBCs contribute less DNA than contaminating WBCs. As a consequence, heterotopic cell interactions during FACS have greater weight on the DNAm profiles of erythroid cells than of WBCs (in this figure, T cells), which are all nucleated. This illustrates how the relative impact of cross-contamination during FACS on DNAm can be much greater for nRBCs than for WBCs. However, WBC DNAm can also be strongly affected in a subset of cases where overall nRBC proportion is high

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