Methylome profiling reveals functions and genes which are differentially methylated in serrated compared to conventional colorectal carcinoma

Table 3 Differences in percentage of methylation in FOXD2 3′ UTR for the two groups defined with each binary variable

Variable	n1	n2	Mean1	Mean2	Eequal variances	Difference (1–2)	gl	t	p values
Source	31 normal	90 tumoural	32.35	52.69	No	−20.35	112	−7.094	<0.001
Nationality	98 Spanish	23 Finnish	47.62	46.89	No	0.72	29	.128	0.899
Sex	32 female	59 male	47.98	46.96	Yes	1.02	119	.262	0.794
M^a	106 0 s	12 1 s	47.38	50.69	Yes	−3.31	116	−.517	0.606
KRAS	85 native	34 mutated	44.89	53.74	Yes	−8.85	117	−2.095	0.038
BRAF	100 native	18 mutated	44.93	62.11	No	−17.18	48	−5.247	<0.001
PI3K exon10^a	90 native	5 mutated	46.91	58.55	Yes	−11.65	93	−1.254	0.213
PI3K exon20^a	92 native	3 mutated	46.91	66.37	No	−19.47	3	−3.692	0.038
MSI	102 MSS	16 MSI	44.65	63.58	No	−18.93	70	−6.887	<0.001

^aWhen the sample size is <15 in any of the groups, t test should not be interpreted. The Mann-Whitney’s U test was used for the variables M, PI3K exon10 and PI3K exon20. No significant differences were found for any of them