Fig. 5

MiR-101 over-expression reduces colony formation and anchorage-independent growth capabilities in eRMS cells. RD (a) and JR1 (b) cells were infected with pS-pre-miR-101 or control pS- retrovirus and, 72 h later, seeded to examine their clonogenic capability 2 weeks post seeding (see “Methods” section). Histograms depict the number of colonies per plate from four independent experiments. Representative pictures of stained colonies were shown on the right. RD (c) and JR1 (d) cells, infected as in (a) and (b), were seeded on soft agar for an anchorage-independent growth assay. Colonies were visible. Histograms depict the number of colonies per plate after 4 weeks of incubation, calculated as means ± SD from four independent experiments. Columns, means; bars, SD. *P < 0.05, **P < 0.01 (Student’s t-test)