Fig. 3
MiR-101 over-expression reduces EZH2 levels and cell proliferation in eRMS cells. RT-qPCR analysis of mature a miR-101 and b EZH2 in RD and JR1 cells 72 h post infection with pS-pre-miR-101 or control pS- retrovirus. Data were normalized using snoU6 and GAPDH levels respectively and expressed as fold increase over control (pS-, 1 arbitrary unit). Columns, means; bars, SD. Results from three independent experiments are shown. *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t-test). c Western blots showing EZH2 and p21Cip1 levels in RD and JR1 cells 72 h post infection with pS-pre-miR-101 or control pS- retrovirus. Total α-tubulin and GAPDH were used as loading controls. Representative of three independent experiments. d Flow cytometry analysis after propidium iodide (PI) staining 72 h post infection with pS-pre-miR-101 or control pS- retrovirus on RD and JR1 cells was performed. Ten thousand events per sample were acquired. The histogram depicts the fold change of cells in the G1, S, and G2 phases after normalization to the percentage of GPF-positive cells for each sample. Results are means ± SD of two independent experiments