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Fig. 1 | Clinical Epigenetics

Fig. 1

From: MicroRNA-101 is repressed by EZH2 and its restoration inhibits tumorigenic features in embryonal rhabdomyosarcoma

Fig. 1

MiR-101 and EZH2 levels are inversely expressed in embryonal rhabdomyosarcoma (RMS) patients and cell lines compared to their controls. a Levels of mature miR-101 (left panel) and EZH2 (right panel) were determined by RT-qPCR in primary embryonal rhabdomyosarcoma (eRMS) samples (black and grey bars, respectively) and in normal skeletal muscles (M1-4) used as control tissues (white bars). Values normalized to snoU6 or GAPDH levels (respectively) were expressed as fold increase over M1 control tissue (1 arbitrary unit). b RT-qPCR of miR-101 (left panel) and EZH2 (right panel) in eRMS cell lines (RD, RD18, RUCH2, and JR1; black and grey bars, respectively) and normal skeletal muscle cells (SKMC) cultured in either growth medium (GM) or differentiating medium (DM) (as described in “Methods” section) were normalized to snoU6 or GAPDH levels, respectively, and were expressed as fold increase over SKMC GM cells (1 arbitrary unit). Two independent measurements were done in duplicate

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