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Fig. 4 | Clinical Epigenetics

Fig. 4

From: The GALNT9, BNC1 and CCDC8 genes are frequently epigenetically dysregulated in breast tumours that metastasise to the brain

Fig. 4

Reduced expression of GALNT9, CCDC8 or BNC1 increases the invasive potential of breast cancer cell lines. Trans-well invasion assays were carried out following the knockdown of of GALNT9, CCDC8 or BNC1 in breast cancer cell lines. The invasive capacity of these cells was compared with the same cell lines transfected with control siRNA oligos (control). The numbers of cells that had invaded a matrigel-coated micropore membrane was determined colourimetrically 48 h after initial seeding. a MDA-MB231 cell lines transfected with siRNA oligos against GALNT9, b T47D cell lines transfected with siRNA oligos against CCDC8 and c MCF7 cell lines transfected with siRNA oligos against BNC1 exhibited a statistically significant increase in invasiveness compared to negative control siRNA transfected cells. p = 0.025 (GALNT9), p = 0.021 (CCDC8) and p = 0.001 (BNC1). Invasive potential was calculated as a percentage increase above that observed for the control cells (% invasion)

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