Figure 5

Pyrosequencing analysis of DNA methylation at the MEG3 differentially methylated region (DMR). Methylation of the MEG3 DMR according to quantitative bisulfite pyrosequencing in (A) 5 benign bladder tissue samples, (B) 4 leukocyte samples, (C-D) 20 urothelial cancer tissues, (E) exceptional uniformly hypermethylated (BT58) and hypomethylated samples (cancer cell line HT1376, cancer tissues BT16, BT28). (F, G) Average methylation of the MEG3 DMR in 5 normal urothelial cell cultures and 12 urothelial cancer cell lines. (H) Comparison of methylation at individual CpG positions in urothelial cancer tissues (light gray) compared to benign bladder tissues (dark gray); P values were obtained by student’s T-test. Please note that the number of lines in the graphs may appear lower than the respective ‘n’ due to overlays between samples with very similar values.