Figure 2From: Concomitant downregulation of the imprinted genes DLK1 and MEG3 at 14q32.2 by epigenetic mechanisms in urothelial carcinomaDLK1 and MEG3 expression in urothelial tissues and cells. Quantitative reverse polymerase chain reaction (RT-PCR) analysis of gene expression relative to the reference gene TBP. (A, C) Boxplot representations of DLK1 (P = 0.004) and MEG3 expression (P = 0.01) in 11 benign (BN) versus 30 cancerous (BT) urothelial tissue samples. (B, D) DLK1 and MEG3 expression in cultured normal urothelial cells (UP) and urothelial carcinoma cell lines (papillary: BC61, BFTC905, J82, RT4, RT112, SW1710; invasive: 5637, 639v, 647v, HT1376, SD, T24, VmCub1, Umuc3) in comparison to HepG2 hepatoma cells. Statistical comparisons between benign bladder and tumor bladder tissue expression were made by the Mann-Whitney U Test with SPSS 21.Back to article page